Bibliografía sobre Células Madre

Bibliografía sobre Células Madre

Inyecciones de Concentrados de Médula Osea

Sampson S, Botto-Van Bemden A, Aufiero D
Autologous Bone Marrow Concentrate: Review and Application of a Novel Intra-Articular Orthobiologic for Cartilage Disease
The Physician and Sports Medicine. 2013 Sep
Jae-Do Kim, Gun Woo Lee, Gu Hee Jung, Cheung Kue Kim, Taehun Kim, Jin Hyung Park, Seong Sook Cha, Young-Bin Yoo
Clinical Outcome of Autologous Bone Marrow Aspirates Concentrate Injection in Degenerative Arthritis of the Knee
European journal of Orthopaedic Surgery and Traumatology. 2014 Jan
Hauser RA, Orlofsky A.
Regenerative injection therapy with whole bone marrow aspirate for degenerative joint disease: a case series.
Clin Med Insights Arthritis Musculoskelet Disord. 2013 Sep 4

Células Regenerativas – Artículos de Revisión

Hernigou P, Homma Y, Flouzat Lachaniette CH, Poignard A, Allain J, Chevallier N, Rouard H.
Benefits of small volume and small syringe for bone marrow aspirations of mesenchymal stem cells.
Int Orthop. 2013 Jul 24.
Christian Hendrich, Engelmaier Franz, Gerhart Waertel, Rolf Krebs, and Marcus Jager.
Safety of autologous bone marrow aspiration concentrate transplantation: initial experiences in 101 patients.
Orthop Rev (Pavia). 2009 October 10; 1(2): e32. Doi: 10.4081/or.2009.e32
Hollander AP, Dickinson SC, Kafienah W
Stem cells and cartilage development: complexities of a simple tissue.
Stem Cells 2010, 28:1992-1996.
Vinatier C, Bouffi C, Merceron C, Gordeladze J, Brondello JM, Jorgensen C, Weiss P, Guicheux J, Noel D
Cartilage tissue engineering: towards a biomaterial assisted mesenchymal stem cell therapy.
Curr Stem Cell Res Ther 2009, 4:318-329.
Mahmoudifar N, Doran PM
Chondrogenesis and cartilage tissue engineering: the longer road to technology development.
Trends Biotechnol 2012, 30:166-176
Andre F. Steinart, Lars Rackwitz, Fabian Gilbert, Ulrich Noth, Rocky S Tuan
Concise Review: The Clinical Application of Mesenchymal Stem Cells for Musculoskeletal Regeneration: Current Status and Perspectives.
Stem Cells Translational Medicine 2012; 1:237-247
Hass R, Kasper C, Bohm S, Jacobs R
Different populations and sources of human mesenchymal stem cells (MSC): a comparison of adult and neonatal tissue-derived MSC.
Cell Commun Signal 2011, 9:12
Caplan AI
Adult mesenchymal stem cells for tissue engineering versus regenerative medicine.
J Cell Physiol 2007, 213:341-347.
Prockop DJ
Marrow stromal cells as stem cells for nonhematopoietic tissues.
Science 1997, 276:71-74.
Romih, et al
“The Vertibral Interbody Grafting Site’s Low Concentration in Osteogenic Progenitors can Greatly Benefit from Addition of Iliac Crest Bone Marrow” European Spine Journal; 14:645-648; 2005
Poole AR
Cartilage in health and disease. In Arthritis and Allied conditions: A Textbook of Rheumatology. 13th edition. Edited by Koopman WS. Baltimore
Lipincott Williams and Wilkins; 1997: 255-308
Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR
Multilineage potential of adult human mesenchymal stem cells.
Science 1999,284:143-147.
Wynn RF, Hart CA, Corradi-Perini C, O’Neill L, Evans CA, Wraith JE, Fairbairn LJ, Bellantuono I
A small proportion of mesenchymal stem cells strongly expresses functionally active CXCR4 receptor capable of promoting migration to bone marrow.
Blood 2004, 104:2643-2645.

Células Mesenquimales – Generalidades

Weiss S, Hennig T, Bock R, Steck E, Richter W
Impact of growth factors and PTHrP on early and lage chondrogenic differentiation of human mesenchymal stem cells.
J Cell Physiol 2010, 223:84-93.
Aggarwal S, Pittenger MF
Human mesenchymal stem cells modulate allogeneic immune cell responses.
Blood 2005, 105:1815-1822.

Médula Osea “in vivo” (ciencias básicas)

Barry F, Boynton RE, Liu B, Murphy JM
Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components.
Exp Cell Res 2001, 268:189-200.
Sekiya I, Larson BL, Vuoristo JT, Reger RL, Prockop DJ
Comparison of effect of BMP-2, -4, and -6 on in vitro cartilage formation of human adult stem cells from bone marrow stroma.
Cell Tissue Res 2005, 320:269-276.
Dar A, Goichberg P, Shinder V, Kalinkovich A, Kollet O, Netzer N, Margalit R, Zsak M, Nagler A, Hardan I, Resnick I, Rot A, Lapidot T
Chemokine receptor CXCR4-dependent inernalization and resecretion of functional chemokine SDF-1 by bone marrow endothelial and stromal cells.
Nat Immunol 2005, 6:1038-1046
Tohru Minamino, Haruhiro Toko, Kaoru Tateno, Toshio Nagai, Issei Komuro.
Peripheral-blood or bone-marrow mononuclear cells for therapeutic angiogenesis?
The lancet, Volume 360, Issue 9350, Pages 2083-2084, 21 December 2002

Modelo Animal

R K W Smith
Stem Cell Therapy for Tendinopathy: Lessons from Large Animal Model
Br J Sports Med 2013;47:e2 doi:10.1136/bjsports-2013-092459.42
Renzi S, Riccò S, Dotti S, Sesso L, Grolli S, Cornali M, Carlin S, Patruno M, Cinotti S, Ferrari M.
Autologous bone marrow mesenchymal stromal cells for regeneration of injured equine ligaments and tendons: A clinical report.
Res Vet Sci. 2013 Feb 16. pii: S0034-5288(13)00019-2. doi: 10.1016/j.rvsc.2013.01.017
Mokbel AN, El Tookhy OS, Shamaa AA, Rashed LA, Sabry D, El Sayed AM.
Homing and reparative effect of intra-articular of autologous mesenchymal stem cells in osteoarthritis animal model.
BMC Musculoskelet Disord. 2011 Nov 15; 12:259.
Sato M, Uchida K, Nakajima H, Miyazaki T, Guerrero AR, Watanabe S, Roberts S, Baba H.
Direct transplantation of mesenchymal stem cells into the knee joints of Hartley strain guinea pigs with spontaneous osteoarthritis.
Arthritis Res Ther. 2012 Feb 7;14(1):R31
Alfaqeh H, Norhamdan MY, Chua KH, Chen HC, Aminuddin BS, Ruszymah BH
Cell based therapy for osteoarthritis in a sheep model: gross and histological assessment.
Med J Malaysia 2008, 63(Suppl A):37-38.
Grigolo B, Lisignoli G, Desando G, Cavallo C, Marconi E, Tschon M, Giavaresi G, Fini M, Giardino R, Facchini A
Osteoarthritis treated with mesenchymal stem cells on hyaluronan-based scaffold in rabbit.
Tissue Eng Part C Methods 2009, 15:647-658.
Murphy JM, Fink DJ, Hunziker EB, Barry FP
Stem cell therapy in a caprine model of osteoarthritis.
Arthritis Rheum 2003, 48:3464-3474.
Wang W, Li B, Yang J, Xin L, Li Y, Yin H, Qi Y, Jiang Y, Ouyang H, Gao C
The restoration of full-thickness cartilage defects with BMSCs and TGF-beta 1 loaded PLGA/fibrin gel constructs.
Biomaterials 2010, 31:8964-8973
Mauro Krampera, Sarah Glennie, Julian Dyson, Diane Scott, Ruthline Laylor, Elizabeth Simpson, and Francesco Dazzi.
Bone marrow mesenchymal stem cells inhibit the response of naïve and memory antigen-specific T cells to their cognate peptide.
Blood May 1, 2003 vol. 101 no. 9 3722-3729
Connelly JT, Wilson CG, Levenston ME
Characterization of proteoglycan production and processing by chondrocytes and BMSCs in tissue engineered constructs
Osteoarthr Cartil 2008, 16:1092-1100.
Fortier LA, Potter HG, Rickey EJ, Schnabel LV, Foo LF, Chong LR, Stokol T, Cheetham J, Nixon AJ.
Concentrated bone marrow aspirate improves full-thickness cartilage repair compared with microfracture in the equine model.
J Bone Joint Surg Am. 2010 Aug 18; 92(10):1927-37
Bouffi C, Thomas O, Bony C, Giateau A, Venier-Julienne MC, Jorgensen C, Montero-Menei C, Noel D
The role of pharmacologically active microcarriers releasing TGF-beta3 in cartilage formation in vivo by mesenchymal stem cells.
Biomaterials 2010, 31:6485-6493.

Artrosis – Artículos de Revisión

Niall A Smyth, Christopher D Murawski, Amgad M Haleem, Charles P Hannon, Ian Savage-Elliott, John G Kennedy
Establishing proof of concept: Platelet-rich plasma and bone marrow aspirate concentrate may improve cartilage repair following surgical treatment for osteochondral lesions of the talus
World J Orthop 2012 July 18; 3(7): 101-108
Kevin J. Campbell, Robert E. Boykin, Coen A. Wijdicks, J. Erik Giphart, Robert F. LaPrade, Marc J. Philippon
Treatment of a hip capsular injury in a professional soccer player with platelet-rich plasma and bone marrow aspirate concentrate therapy. Knee Surg Sports Traumatol Arthrosc.
Goldring MB, Goldring SR
Articular cartilage and subchondral bone in the pathogenesis of osteoarthritis.
Ann N Y Acad Sci 2010, 1192:230-237.
Buckwalter JA, Saltzman C, Brown T
The impact of osteoarthritis: implications of research.
Clin Orthop Relat Res 2004, (427 Suppl):S6-S15
Koelling S, Miosge N
Stem cell therapy for cartilage regeneration in osteoarthritis.
Expert Opin Biol Ther 2009, 9:1399-1405.
Charles J. Malemud and Victor M. Goldberg
Future Directions for Research and Treatments of Osteoarthritis
Frontiers in Bioscience 4, d762-771, October 15, 1999
Chevalier X
Intraarticular treatments for osteoarthritis: new perspectives
Curr Drug Targets 2010, 11:546-560.
Pawan K. Gupta, Anjan K. Das, Anoop Chullikana and Anish S. Majumdar
Mesenchymal stem cells for cartilage repair in osteoarthritis.
Stem Cell Research & Therapy 2012, 3:25 doi: 10.1186/scrt116
Chen FH, Tuan RS
Mesenchymal stem cells in arthritic diseases.
Arthritis Res Ther 2008, 10:223.
Niemeyer P, Krause U, Kasten P, Kreuz PC, Henle P, Sudkam NP, Mehlhorn A
Mesenchymal stem-cell based HLA-independent cell therapy for tissue engineering of bone and cartilage.
Curr stem Cell Res Ther 2006, 1:21-27
Singh A Jasvinder
Stem Cells and other innovative intra-articular therapies for osteoarthritis: What does the future hold?
BMC Medicine 2012, 10:44
Wakitani S, Nawata M, Tensho K, Okabe T, Machida H, Ohgushi H
Repair of articular cartilage defects in the patello-femoral joint with autologous bone marrow mesenchymal cell transplantation: three case reports involving nine defects in five knees.
J Tissue Eng Regen Med 2007, 1:74-79
Nejadnik H, Hui JH, Feng Choong EP, Tai B-C, Lee EH
Autologous bone marrow-derived mesenchymal stem cells versus autologous chondrocyte implantation: an observational cohort study.
Am J Sports Med 2010, 38:1110-1116.
Davatchi F, Abdollahi BS, Mohyeddin M, Shahram F, Nikbin B
Mesenchymal stem cell therapy for knee osteoarthritis. Preliminary report of four patients.
Int J Rheum Dis 2011, 14:211-215.
Mohsen Emadedin MD, Naser Aghdmani MD PhD, Leilar Taghiyar MSc, Roghayeh Fazeli MD, Reza Moghadasali MSc, et al
Intra-articular injection of Autologous Mesenchymal Stem Cells in Six patients with Knee Osteoarthritis
Arch Iran Med. 2012; 15(7): 422-428.
Kasemkijwattana C, Hongeng S, Kesprayura S, Rungsinaporn V, Chaipinyo K, Chansiri K
Autologous bone marrow mesenchymal stem cells implantation for cartilage defects: two case report.
J Med Assoc Thai 2011, 94:395-400.
Centeno CJ, Busse D, Kisiday J, Keohan C, Freeman M, Karli D
Increased knee cartilage volume in degenerative joint disease using percutaneously implanted, autologous mesenchymal stem cells.
Pain Physician 2008, 11:343-353.
Veronesi F, Giavaresi G, Tchon M, Borsari V, Nicoli Aldini N, Fini M.
Clinical Use of Bone Marrow, Bone Marrow Concentrate, and Expanded Bone Marrow Mesenchymal Stem Cells in Cartilage Disease.
Stem Cells Dev. 2012 Nov 16. PMID: 23030230

Lesiones de Cartílago

Filardo G, Madry H, Jelic M, Roffi A, Cucchiarini M, Kon E.
Mesenchymal stem cells for the treatment of cartilage lesions: from preclinical findings to clinical application in orthopaedics.
Knee Surg Sports Traumatol Arthrosc. 2013 Jan 11.
Veronesi F, Giavaresi G, Tchon M, Borsari V, Nicoli Aldini N, Fini M.
Clinical Use of Bone Marrow, Bone Marrow Concentrate, and Expanded Bone Marrow Mesenchymal Stem Cells in Cartilage Disease.
Stem Cells Dev. 2012 Nov 16. PMID: 23030230
Niall A Smyth, Christopher D Murawski, Amgad M Haleem, Charles P Hannon, Ian Savage-Elliott, John G Kennedy
Establishing proof of concept: Platelet-rich plasma and bone marrow aspirate concentrate may improve cartilage repair following surgical treatment for osteochondral lesions of the talus
World J Orthop 2012 July 18; 3(7): 101-108
Kuroda R, Ishida K, Matsumoto T, Akisue T, Fujioka H, Mizuno K, Ohgushi H, Wakitani S, Kurosaka M
Treatment of a full-thickness articular cartilage defect in the femoral condyle of an athlete with autologous bone-marrow stromal cells.
Osteoarthr Cartil 2007, 15:226-231.
Haleem AM, Singergy AAE, Sabry D, Atta HM, Rashed LA, Chu CR, El Shewy MT, Azzam A, Abdel Aziz MT
The clinical use of human culture-expanded autologous bone marrow mesenchymal stem cells transplanted on platelet-rich fibrin glue in the treatment of articular cartilage defects: a pilot study and preliminary results.
Cartilage 2010, 1:253-261.


Pascual-Garrido C, Rolón A, Makino A.
Treatment of chronic patellar tendinopathy with autologous bone marrow stem cells: a 5-year-followup.
Stem Cells Int. 2012;2012:953510. doi: 10.1155/2012/953510. Epub 2011 Dec 18.

Lesiones Meniscales

Duygulu F, Demirel M, Atalan G, Kaymaz FF, Kocabey Y, Dülgeroğlu TC, Candemir H.
Effects of intra-articular administration of autologous bone marrow aspirate on healing of full-thickness meniscal tear: an experimental study on sheep.
Acta Orthop Traumatol Turc. 2012;46(1):61-7.

Lesiones Cápsulo-Ligamentosas

Kevin J. Campbell, Robert E. Boykin, Coen A. Wijdicks, J. Erik Giphart, Robert F. LaPrade, Marc J. Philippon
Treatment of a hip capsular injury in a professional soccer player with platelet-rich plasma and bone marrow aspirate concentrate therapy.
Knee Surg Sports Traumatol Arthrosc.

Osteonecrosis de Cadera o Rodilla

Pak J
Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series
J Med Case Rep 2011, 5:296.
Gangji V, Hauzeur JP, Matos C, De Maertelaer V, Toungouz M, Lambermont M.
Treatment of osteonecrosis of the femoral head with implantation of autologous bone-marrow cells: A pilot study
J Bone Joint Surg Am. 2004 Jun; 86-A(6):1153-60.

Consolidación Osea

Vulcano E, Murena L, Falvo DA, Baj A, Toniolo A, Cherubino P.
Bone marrow aspirate and bone allograft to treat acetabular bone defects in revision total hip arthroplasty: preliminary report.
Eur Rev Med Pharmacol Sci. 2013 Aug;17(16):2240-9.
Gessman J, Koller M, Godry H, Schildhaur T, Seyhold D, Department of General and Trauma Surgery and Department of Surgical Research, BG University Hospital
Regenerate Augmentation with bone marrow concentrate after traumatic bone loss.
Orthopedic Reviews 2012; volume 4:e14
Ph. Hernigou, MD; A. Poignard, MD; F. Beaujean, MD; H. Rouard, MD.
Percutaneous Autologous Bone-Marrow Grafting for Nonunions: Influence of the Number and Concentration of Progenitor Cells.
The Journal of Bone and Joint Surgery. 2005; 87:1430-1437

Isquemia de Miembros

Yuyama, et al
Therapeutic Angiogenesis for Patients with Limb Ischemia by Autologous Transplantation of Bone Marrow Cells: A Pilot Study and Randomized Controlled Study
The Lancet; 360:427-435; 2002
Nishimoto S, Kawai K, Tsumano T, Fukuda K, Fujiwara T, Kakibuchi M.
Impacts of bone marrow aspirate and peripheral blood derived platelet-rich plasma on the wound healing in chronic ischaemic limb.
Plast Surg Hand Surg. 2013 Apr 26.

Tratamiento Integral de la Artrosis

Radiografía de artrosis de rodilla



La artrosis es una de las principales causas de discapacidad del sistema musculo-esquelético en las sociedades occidentales y una de las mayores cargas económicas de los sistemas sanitarios. De manera progresiva y de forma crónica provoca dolor y perdida de la función de las articulaciones, reduciendo, a veces de forma dramática, la calidad de vida de los pacientes y su capacidad para trabajar.

La artrosis es el tipo de artritis más común que existe y consiste básicamente en un la presencia de dolor articular y alteración de la función de la articulación.

Se caracteriza principalmente por el desgaste y la degeneración del cartílago articular, la presencia de inflamación dentro de la articulación, en la membrana sinovial que la recubre por dentro, y el deterioro progresivo en los huesos y demás tejidos que rodean a la articulación.


El cartílago articular actúa como una almohadilla, absorbiendo las cargas mecánicas y facilitando un movimiento de fricción en las articulaciones, lo que permite el deslizamiento de unos huesos sobre otros, dando origen al movimiento articular. La lesión del cartílago articular provoca la pérdida de la lubricación natural, aumentando el roce de las superficies articulares de los huesos y la formación de excrecencias óseas u osteofitos. En algunos casos los fragmentos de cartílago pueden desprenderse y permanecer flotando en el interior de la articulación, provocando irritación articular, inflamación y mayor daño estructural.


Lo mas frecuente es la artrosis primaria como resultado de la edad y el desgate asociado con el empleo de las articulaciones durante años, asociándose con frecuencia el sobrepeso y/o la presencia de deformidades angulares, especialmente al nivel de la rodilla. Se trata, por tanto, del envejecimiento natural de las articulaciones.

Otras posibles causas son:

  • Lesiones o traumatismos previos de las articulaciones.
  • Intervenciones previas sobre las articulaciones (Ej: Meniscos)
  • Infecciones.
  • Enfermedades inflamatorias crónicas (Ej: artritis reumatoide)


Los principales síntomas de la artrosis son dolor, rigidez e inflamación de las articulaciones. La articulación afecta suele presentar limitación de la movilidad y puede existir también hipersensibilidad en la zona y deformidades óseas asociadas (muchas veces dichas deformidades son también las causantes iniciales de la artrosis). A veces la articulación puede provocar crujidos, lo cual se denomina crepitación. A su vez, cuando la articulación está muy afectada, pueden aparecer deformidades secundarias y progresivas

Tratamiento de la artrosis


¿Cuál es su tratamiento?

No existe, todavía, una terapia médica que permita curar la enfermedad.


El tratamiento debe ser global y escalonado (de menos a mas en función de la gravedad de la artrosis). Habitualmente se combina el escalón 1 con otros escalones.


  • Hábitos: modificación de actividades y control de peso
  • Soportes y ortesis (restablecer la estabilidad articular)
  • Fisioterapia:
  • Ejercicio moderado
  • Frio / Calor
  • Acupuntura
  • Tens (Transcutaneous Electrical Nerve Stimulation)
  • Ultrasonidos (tratamiento de lesiones inflamamatorias asociadas (tendinitis, bursitis, etc.).
  • Suplementos nutricionales (mantenimiento)
  • Colágeno hidrolizado
  • Magnesio
  • Cúrcuma


  • Analgésicos (Paracetamol, Metamizol, Tramadol,…)
  • Cremas antinflamatorias (Capsaicina,…)
  • Antinflamatorios no esteroideos (AINES) vía oral (Ibuprofeno, Diclofenaco, Desketoprofeno, Celecoxib (Inhibidores Cox-2), …).
  • Corticoides por vía oral o intramuscular (en fases muy agudas de dolor o en enfermedades inflamatorias crónicas).
  • Fármacos “condroprotectores” (sulfato de glucosamina, condroitin sulfato, …)


  • Inyecciones intra-articulares de corticoides
  • Inyecciones intra-articulares de ácido hialurónico



  • Artroscopia, desbridamiento y lavado
  • Osteotomías
  • Prótesis articular
  • Parcial
  • Total




Factores de crecimiento. Plasma Rico en Plaquetas. Qué es y cómo se obtiene.

Regeneración de Tejidos con Células Madre Mesenquimales.

Plasma Rico en Plaquetas. Factores de Crecimiento. Tipos y Formas de obtención.



Curso sobre CELULAS MADRE procedentes de Médula Osea y Grasa

El Dr. Mora asiste al Curso sobre CELULAS MADRE procedentes de Médula Osea y Grasa, los días 9 y 10 de Octubre en Barcelona.